Touchstone Diabetes Center

Metabolic Phenotyping Core

MagPix (Cytokines)

The MagPix is a multiplexing machine that uses magnetic bead mapping technology to obtain concentrations of multiple cytokines in samples.

What is bead mapping?

Luminex color-codes tiny beads, called microspheres, into 500 distinct sets. Each bead set can be coated with a reagent specific to a particular bioassay, allowing the capture and detection of specific analytes from a sample. Inside the Luminex analyzer, a light source excites the internal dyes that identify each microsphere particle, and also any reporter dye captured during the assay. Many readings are made on each bead set, which further validates the results. Using this process, xMAP Technology allows multiplexing of up to 500 unique bioassays within a single sample, both rapidly and precisely. The MAGPIX® system is based on the principles of fluorescence imaging. Lasers and Photo Multiplying Tubes (PMTs) are replaced with Light Emitting Diodes (LEDs) and a CCD camera to deliver a cost effective, compact, and reliable multiplexing platform.

© 2011 Luminex Corporation.  xMap and MagPix are trademarks of Luminex Corporation.

What cytokines are available?

The Mouse Metabolic Phenotyping Core offers a large selection of cytokines for human, mouse and rat specimens.  Here is a complete list of what we have available:

Assays Human Mouse Rat
IL-1α
IL-1β
IL-1ra    
IL-2
IL-2Rα    
IL-3  
IL-4
IL-5
IL-6
IL-7  ●  
IL-8    
IL-9
IL-10
IL-12 (p40)  
IL-12 (p70)
IL-13
IL-15  
IL-17A
IL-18
IL-20
IL-21
IL-23
IL-28a
IL-33/NF-HEV (mature)
FGF-2  
Flt-3 ligand
CTACK    
Eotaxin
EGF    
EPO
Fractalkine ●   
G-CSF
GM-CSF
GRO    
GRO/KC    
HGF    
ICAM-1    
IFN-α2    
IFN-γ
IP-10  ●
KC    
LIF  
LIX
MCP-1 (MCAF)
MCP-2
MCP-3    
MCP-4
MCP-5
MDC
M-CSF  
MIG  
MIP-1α
MIP-1β  
MIP-2    
β-NGF    
PDGF-BB  
RANTES
SCF    
sCD40L    
SCGF-β    
SDF-1α    
TARC
TGF-α    
TNF-α
TNF-β    
TPO
TSLP
TRAIL    
VCAM-1    
VEGF

Usage Fee = Price of the Plate + $200 for labor

(up to 36 samples per plate in duplicates)

To know what sample volume to provide, please contact the core with a list of cytokines you are interested in testing.

* Not available for single-plexing.

magpix

Please contact:Ruth Gordillo /Eva (Athena) Mason

 

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