Metabolic Phenotyping Core
XF-24 Extracellular Flux Analyzer
XF-24(3) Extracellular Flux Analyzer (Seahorse Bioscience)
The XF-24 analyzer simultaneously measures the Oxygen Consumption Rate (OCR) and the Extracellular Acidification Rate (ECAR) of cultured cells, i.e. the instrument allows to measure mitochondrial function in intact cells utilizing real-time bioenergetics - the rate of aerobic respiration and lactic acid production via glycolysis, respectively.
The plate accommodates 24 individual wells, and up to 4 different test compounds (for example, 2-DG, FCCP, 2,4-DNP, Rotenone) can be injected separately into each well.
The instrument detects real-time alterations in cellular metabolism (with a CO2/O2/H ; three Analyte Detection Capability). Furthermore, the system performs label-free, non-destructive measurements, which allow the cultured cells to be utilized for further assays.
$360 per experiment
Users will provide cells and offer basic info for experimental design or goal of experiment.
Core facility will supply all XF consumables.
Core facility will plate and grow cells for XF assay.
Core facility will assist with proper experiment design and create XF assay template.
Core facility will perform media exchange and run assay in XF instrument.
Core facility will prepare XF cartridge with compounds (oligo > FCCP > Rotenone) for typical bioenergetic profile experiment.
Core facility will perform data analysis, generation of data summary and send by email.
XF assay takes at least 4 hours. So data will be provided by the next day.