Touchstone Diabetes Center

Metabolic Phenotyping Core

XF-24 Extracellular Flux Analyzer

XF-24(3) Extracellular Flux Analyzer (Seahorse Bioscience)

The XF-24 analyzer simultaneously measures the Oxygen Consumption Rate (OCR) and the Extracellular Acidification Rate (ECAR) of cultured cells, i.e. the instrument allows to measure mitochondrial function in intact cells utilizing real-time bioenergetics - the rate of aerobic respiration and lactic acid production via glycolysis, respectively.

The plate accommodates 24 individual wells, and up to 4 different test compounds (for example, 2-DG, FCCP, 2,4-DNP, Rotenone) can be injected separately into each well.

The instrument detects real-time alterations in cellular metabolism (with a CO2/O2/H ; three Analyte Detection Capability). Furthermore, the system performs label-free, non-destructive measurements, which allow the cultured cells to be utilized for further assays.

Usage Fees

$360 per experiment

XF assay takes at least 4 hours. So data will be provided by the next day.

 

Xf-24

For questions and scheduling, please contact: Ruth Gordillo / Duyen Do

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